hollow fibre systems  (FiberCell Systems)

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Evaluation of temocillin efficacy against KPC-2-producing Klebsiella pneumoniae isolates in a hollow-fibre infection model

doi: 10.1093/jac/dkae027

Figure Lengend Snippet: Pharmacokinetic profiles in the HFIM after dosages of temocillin 2 g q8h. (a) The solid line shows the predicted concentrations and open circles the observed concentrations. (b) Scatterplot showing observed versus predicted antimicrobial concentrations. The open circles and the solid line represent individual observed-predicted data points and the linear regression of observed/predicted values, respectively.

Article Snippet: Molecular typing and characterization of the antimicrobial resistance determinants of the clinical isolates and those derived from the hollow-fibre infection model (HFIM) experiment were performed by WGS using the MiSeq system (Illumina, San Diego, CA, USA).

Techniques:

Journal: Journal of Extracellular Biology

Article Title: Scalable purification of extracellular vesicles with high yield and purity using multimodal flowthrough chromatography

doi: 10.1002/jex2.138

Figure Lengend Snippet: SEC cannot separate EVs from contaminants in bioreactor harvests, while MFC removes large quantities of free protein. (a) UV chromatogram of EVs purified with SEC from low scale 2D culture, where ‘a’ represents the EV/particle peak, and ‘b’ represents smaller contaminants such as free protein. (b) UV chromatogram of three consecutive passes of the same large scale bioreactor culture through SEC. (c) UV chromatogram of purified EV samples from the Capto Core purified by SEC to separate any remaining free protein or contaminants from the EV peak, where ‘a’ represents the EV peak, ‘b’ the contaminant peak, and ‘c’ represents the right shoulder attributed to DMEM constituents (Figure ). (d) Protein concentration in EV samples isolated using different Capto Core columns as determined by a microBCA protein assay. (e) UV chromatogram of a representative EV purification through CC700 MFC from large scale bioreactor culture.

Article Snippet: HEK293T cells (ATCC) cultured in DMEM (Gibco) + 10% FBS (Gibco) + 1% 100x antibiotic/antimycotic solution (Sigma Aldrich) were expanded to 1 × 10 8 cells in 15 cm dishes and seeded into the extra‐capillary space (ECS) of FiberCell Systems C2011 20 kDa molecular weight cut‐off (MWCO) hollow‐fibre bioreactor (FiberCell Systems).

Techniques: Purification, Protein Concentration, Isolation

Journal: Membranes

Article Title: Recent Advances of Polymeric Membranes in Tackling Plasticization and Aging for Practical Industrial CO 2 /CH 4 Applications—A Review

doi: 10.3390/membranes12010071

Figure Lengend Snippet: Summary of polymer-modification improvement strategies.

Article Snippet: In 1979, Monsanto fabricated the first membrane separation system using PSF hollow fibres [ ], and it was later commercialized for water removal by Air Products, under the trade name ‘Prism’ [ ].

Techniques: Polymer, Modification, Solubility, Pore Size, Activation Assay, Permeability

Journal: Membranes

Article Title: Recent Advances of Polymeric Membranes in Tackling Plasticization and Aging for Practical Industrial CO 2 /CH 4 Applications—A Review

doi: 10.3390/membranes12010071

Figure Lengend Snippet: Summary of MMM performance for different gas pairs.

Article Snippet: In 1979, Monsanto fabricated the first membrane separation system using PSF hollow fibres [ ], and it was later commercialized for water removal by Air Products, under the trade name ‘Prism’ [ ].

Techniques: